Protocols

pCFD1 – U6:1-sgRNA

Medium level ubiquitous expression of a single Cas9 sgRNA

pCFD2 – U6:2-sgRNA

Low level ubiquitous expression of a single Cas9 sgRNA

pCFD3 – U6:3-sgRNA

High level ubiquitous expression of a single Cas9 sgRNA

pCFD4 – U6:1-sgRNA U6:3-sgRNA

Ubiquitous expression of two Cas9 sgRNAs from medium and high efficiency promoters

pCFD5(w) – U6:3-tRNA:sgRNA(xN)

Ubiquitous expression of one to several Cas9 sgRNAs from high efficiency promoter. Available with white or vermillion transformation marker.

pCFD6 – UAS-tRNA:sgRNA(xN)

Gal4 dependent expression of one to several Cas9 sgRNAs from UAS promoter.