Protocols

General advice

New to fly genome engineering with CRISPR/Cas? Read our thoughts on the different protocols that are available for CRISPR/Cas in Drosophila before setting up your own experiments.

Avoid off-targets

Several different strategies are discussed to minimise the risk of generating off-target mutations.

Make gRNAs

Cloning protocols to generate gRNA expression vectors that can be injected into embryos for direct expression or to make stable transgenic lines. We also have a protocol to produce gRNAs by in-vitro transcription.