Category: CFD2

Differential activity of U6 gRNA plasmids

Fillip Port1, Hui-Min Chen2, Tzumin Lee2 and Simon Bullock1 1: MRC-Laboratory of Molecular Biology, Cambridge, UK 2: Janelia Farm Research Campus, HHMI, Ashburn, USA   Delivery of gRNAs for fly CRISPR/Cas genome engineering is most commonly done by either DNA or RNA injection or by creating transgenic gRNA lines.  Plasmids for both DNA injection and gRNA transgenes use polymerase III…

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