Category: CFD-5

First test of stock CFD-5: UAS-cas9

Hui-Min Chen and Tzumin Lee Janelia Farm Research Campus, HHMI   We expressed UAS-cas9 (stock CDF-5) under the control of a germline Gal4 line and crossed these flies to transgenic flies expressing gRNAs targeting yellow under the control of the U6-3 promoter (pU6-3-gRNA y). The gRNA target site was: GGAGCGATATAGTTGGAGCCAGCTGG. Female offspring of this cross was then crossed to yellow…

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